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KMID : 0379120170450030196
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Korean Journal of Mycology
2017 Volume.45 No. 3 p.196 ~ p.211
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Functional Analysis of Aspergillus nidulans Genes Selected by Proteomic Analysis under Conditions Inducing Asexual Development
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Lim Joo-Yeon
Kang Eun-Hye
Jung Bo-Ri
Park Hee-Moon
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Abstract
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Despite the significance of external environmental factors in differentiation, putative factors involved in differentiation of have not yet been fully understood. A sporulation-specific proteome analysis of in the present study revealed that the expression levels of more than 2,400 proteins were affected under conditions inducing sporulation (0.6 M KCl) compared with normal conditions. Among the proteins with predicted functions, two targets, AN1342 and AN9419, were functionally analyzed using targeted deletion strains and phenotypic observations. For AN1342, because the deletion of the corresponding open reading frame caused a reduction in stalk length during asexual development and in pigment production in liquid culture, the gene was designated as sspA (short stalk & pigment). Deletion of the AN9419 gene, which is predicted to encode alanyl-tRNA synthetase, led to severe growth defects due to alanine auxotrophy and abolishment of asexual reproduction and thus, the gene was designated as .
Despite the significance of external environmental factors in differentiation, putative factors involved in differentiation of have not yet been fully understood. A sporulation-specific proteome analysis of in the present study revealed that the expression levels of more than 2,400 proteins were affected under conditions inducing sporulation (0.6 M KCl) compared with normal conditions. Among the proteins with predicted functions, two targets, AN1342 and AN9419, were functionally analyzed using targeted deletion strains and phenotypic observations. For AN1342, because the deletion of the corresponding open reading frame caused a reduction in stalk length during asexual development and in pigment production in liquid culture, the gene was designated as sspA (short stalk & pigment). Deletion of the AN9419 gene, which is predicted to encode alanyl-tRNA synthetase, led to severe growth defects due to alanine auxotrophy and abolishment of asexual reproduction and thus, the gene was designated as
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KEYWORD
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Aspergillus nidulans, Development, Proteome, Sporulation
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